# 3.2. Reannotation of Probes in Microarrays ### Microarrays are made up of several short-length probes that test different parts of the same transcript, which may or may not be from a protein-coding gene. However, because many platforms were developed years ago, the functional annotation of each transcript may be out of date. Therefore, the selected platforms were re-annotated using more recent versions of databases, such as Gencode (HARROW et al., 2012), Noncode (ZHAO et al., 2016), LNCipedia (VOLDERS et al., 2013) and MiTranscriptome (IYER et al., 2015) (Figure 3.2). Through intersections between genomic coordinates, probes from each platform were confirmed or assigned to new transcripts, such as coding genes, non-coding RNAs, among others. ![Figura 3.2. Fluxograma sobre a reanotação. Fluxograma representando as etapas do processo de reanotação das sondas de microarray. Arquivos estão representados pelas formas em azul claro e programas e processos por formas em azul escuro. Adaptado de Carvalho-Bürger, 2017.](https://lh5.googleusercontent.com/TyFiJCrb1naHrDuIvmjlJdw0eTZq0E-Edi0oh_2oMzhegZA1Fdn8V6-gEFCv9B9f-DfpcIxVyweD0i_VpMKO4NjrZkq0NgLTBOlk-kaIe9ipMimC4AeR3QWFg35lIDGAwq3I_vi5=s0) --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://fmarcon.gitbook.io/aging-sysbio/methods/3.2.-reanotacao-das-sondas-nos-microarrays.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.